Senior Technician

Columbia University
United States, New York, New York
Mar 01, 2025
Job Type: Support Staff - Union Bargaining Unit: SSA Regular/Temporary: Regular End Date if Temporary: Hours Per Week: 35 Standard Work Schedule: Building: Salary Range: $59,845.49 - $59,845.49 The salary of the finalist selected for this role will be set based on a variety of factors, including but not limited to departmental budgets, qualifications, experience, education, licenses, specialty, and training. The above hiring range represents the University's good faith and reasonable estimate of the range of possible compensation at the time of posting. Position Summary The Sadelain Lab is looking for a motivated senior laboratory technician to join their team. This position will focus on CAR T cell studies in murine models. The Sadelain Lab investigates cell engineering for the treatment of cancers and other illnesses, including cancer, neurological diseases, and autoimmune and transplant-related conditions. The lab will be a foundational research group for the Columbia Initiative in Cell Engineering and Therapy (CICET), directed by Dr. Sadelain. Reporting to the Sadelain Lab Manager, the Senior Technician will be responsible for the maintenance and management of the Sadelain mouse colonies, administer T cells, tumor cells and hematopoietic cells to mice, and perform various assays and analyses on cells retrieved from mice. Responsibilities Set-up, maintain and manage a large mouse colony for the Sadelain lab Infuse T cells and hematopoietic cells, establish tumors (cell lines and PDX) by IV or IP administration Perform regular measurement of cell engraftment, persistence and tumor burden by bioluminescence imaging Maintain a breeding colony for mice bearing specific mutations Perform daily lab activities and assist lab members with cell infusions or cell retrieval Extract genomic DNA or prepare retrieved tissues for multi-color FACS analysis Assists in lab research by performing surgical procedures to establish orthotopic tumors Assists in research by doing histology through cryostat sectioning Direct lab contact for ICM (Institute of Comparative Medicine) for any sick mice for the lab Orders mice and serve as the lab liaison for the completing of MTA (Materials Transfer Agreements) when needed Manages and updates protocols used in various disease models Oversee expenses related to animal purchase, husbandry and housing costs Order lab supplies Perform other responsibilities as assigned Minimum Qualifications Requires a Bachelor's Degree and at least 1.5 years of related experience or equivalent in education, training and experience. Role specific competencies and skill requirements Willingness to work with mice Technical accuracy, reliability, and attention to the details of procedural protocols, organizational skills, adherence to safety guidelines and the ability to work in a team Excellent organizational and communication skills and a strong interest in biological sciences Participation in the medical surveillance program: Laboratory animals Potential bloodborne pathogen exposure Must successfully complete applicable systems training requirements Preferred Qualifications Bachelor's degree in biomedical sciences Research experience in a biology lab - wet lab experience Well-versed in molecular biology and basic laboratory techniques Experience in mouse handling including cell infusion procedures and colony maintenance highly preferred Experience in basic histological procedures (i.e. immunohistochemistry) is preferred but not essential Proficient at performing complicated experiments in a timely manner Ability to work independently and collaboratively Strong organizational skills and ability to maintain detailed electronic laboratory records Ability to set up and conduct experiments or activities according to established protocols Other Requirements The candidate selected for this position is required to undergo a drug screening test prior to a final offer of employment being made. Required Documents Cover letter describing experience and motivation for applying to the position CV Information for three reference letter writers, please describe relation to candidate Equal Opportunity Employer / Disability / Veteran Columbia University is committed to the hiring of qualified local residents.