Research Technologist I

Position Description:

Research Technologist I

The Tecle lab in the Department of Microbiology and Immunology at the Medical College of Wisconsin seeks a Research Technologist I. The lab's overarching goal is to understand innate epithelial immune responses to intracellular pathogens. To do this, we investigate the response of the C. elegans intestine to pathogen infection using genetic, cell biology, and biochemical approaches.

Under the supervision of the PI, a successful candidate will support ongoing research projects, assist in laboratory management, and facilitate ongoing collaborations. Independent research projects may be available based on the demonstration of mastery of routine experimental techniques and other skills, including, but not limited to, effective communication, personal management, data recording, and proactiveness. This is an excellent position for an individual who wants to gain 2 to 3 years of research experience in preparation for graduate or medical school. Preference will be given to individuals with previous experience with C. elegans; however, this is not a requirement for a successful hire. The start date for this position is July 1^st, 2025.

Primary Functions

* Perform standard experimental techniques such as PCR, qRT-PCR, plasmids preps, DNA cloning, bacterial transformation, expression and
purification of proteins, gel electrophoresis and CRISPR

* Prepare media and reagents for bacterial and C. elegans work

* Create, organize and maintain laboratory standard operating procedures and databases (strains, plasmids,

genotyping primers, etc)

* Generate transgenic C. elegans strains via microinjection

* Record and compile accurate records and communicate summaries of experimental results.

* Assist with the evaluation, interpretation and assessment of experimental data and quality control of assays.

* Participate in meetings with principal investigator and research staff and evaluate/interpret the validity of data as needed.

* Carry out assigned duties for ordering and maintaining supply of inventories.

* Operate, calibrate and perform routine maintenance on specialized equipment used in research.

* Oversee daily lab operation in the absence of the supervisor or Principal Investigator.

Knowledge - Skills - Abilities

Knowledge of biology, chemistry, mathematics, documentation, and records management.

Data utilization, complex problem solving, critical thinking, resource management, and writing skills.

Preferred Schedule:



M-F 8-5

Position Requirements:

Appropriate experience may be substituted for education on an equivalent basis

Minimum Required Education: Bachelor's degree

Minimum Required Experience: No experience required

Preferred Education: Bachelor's degree

Preferred Experience: C. elegans specific experience outside of required coursework

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MCW as an Equal Opportunity Employer and Commitment to Non-Discrimination


The Medical College of Wisconsin (MCW) is an Equal Opportunity Employer. We are committed to fostering a diverse community of outstanding faculty, staff, and students, as well as ensuring equal educational opportunity, employment, and access to services, programs, and activities, without regard to an individual's race, color, national origin, religion, age, disability, sex, gender identity/expression, sexual orientation, marital status, pregnancy, predisposing genetic characteristic, or military status. Employees, students, applicants or other members of the MCW community (including but not limited to vendors, visitors, and guests) may not be subjected to harassment that is prohibited by law or treated adversely or retaliated against based upon a protected characteristic.

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